An increase in the number of the probiotic population in the flask that contained date seed powder was observed in the first fermentation assay. The culture was grown for 4-6 h before running the experiment. a: AEDSP: aqueous extract of date seed powder; b  MRS-Min : free from Carbon and Nitrogen sources. Share Your PPT File. The highest probiotic Lactobacillus populations were observed in the flask containing the FGDSP with fewer numbers being observed in the DFC flask and a similar quantity observed in the AEDSP flask (Figure 6).In general, the probiotic Lactobacillus seems to utilize DFC as a carbon source, and an increase in the total number of probiotic populations were observed in response to the addition of each of the date seed products DFC, FGDSP and AEDSP over the course of the fermentation period (96 h). All these samples in this assay contained the same amount of AEDSP, using the amount that resulted in optimal growth in the first fermentation assay. The isolation and identification of the probiotic culture from yoghurt were carried out as a modifications of the methods described by (20, 21). What is the significance of transpiration? The short trunk has suckers at the base. Worldwide production of dates was around 6.9 million tonnes in 2004, from which about 863 thousand tonnes of date seeds were disposed of as a waste product (12). The kernels were soaked in distilled water, washed to remove any adhering date flesh, and then dried at 50 °C for two days before being finely ground to1.0 mm in a heavy-duty grinder (Buhler-Miag laboratory disc mill; model DLFU). The DFC exhibited the potential to be used as a novel source of prebiotic by increasing the population of probiotic Lactobacillus paracaseisspparacasei with a concomitant decrease in the pH values of the medium. In a recent study, the aqueous extract of P. dactylifera seeds was able to stimulate endogenous insulin secretion through extra- It was successfully sprouted in 2005 (Sallon et al, 2008). After incubation, five colonies for each sample were selected from MRS plates and subjected to morphological examination, Gram staining and biochemical tests like oxidase and catalase. However, the oldest 14C-dated seed that has grown into a viable plant was Judean date palm (a cultivar of Phoenix dactylifera) seed about 2000 years old, recovered from excavations at Herod the Great’s palace at Masada in Israel. The investigations that were carried out in the extracted dietary fiber concentrates (DFC) demonstrated that the total numbers of probiotic bacteria were increased in the first 72 h of incubation whereas the total bacterial populations decreased after 48 h incubation as shown in. The latter (DFC)may have supported the probiotic Lactobacillus growth especially after 48 h incubation (Figure 4). The commercially available medium was used to recover the probiotic cultures and to enumerate them during this study. Badis, A., Guetarni, D., Moussa-Boudjemaa, B., Henni, D. E., Tornadijo, M. E.,Kihal, M. Identification of cultivable lactic acid bacteria isolated from Algerian raw goat’s milk and evaluation of their technological properties. The fermentation medium used for the third fermentation assay was prepared by using distilled water supplemented by each of the three different date seeds products (Table 1). Which organelle is known as “power house” of the cell? (With Methods)| Industrial Microbiology, How is Cheese Made Step by Step: Principles, Production and Process, Enzyme Production and Purification: Extraction & Separation Methods | Industrial Microbiology, Fermentation of Olives: Process, Control, Problems, Abnormalities and Developments, The best answers are voted up and rise to the top. 2.2. The last fermentation assay was carried out to confirm the results from the first and second fermentation assays and to study the ability of the probiotic Lactobacillus to control the growth foodborne pathogens and to investigate the prebiotic properties of date palm DFC in stimulating probiotic Lactobacillusbacteriocin production was also investigated. The seeds – the oldest ever germinated – were among hundreds discovered in caves and in an ancient palace built by King Herod the Great in the 1st century BC. It is believed that these viable seeds must have been at least 130 years old and probably even 200-400 years old. The date palm (Phoenix dactylifera L.) is an ancient fruit with significant nutritional, medicinal and economic value.Date fruits are composed of a fleshy pericarp and a single seed. Table 5.Enumeration (CFU /ML) of probiotic using DFC, FGDSP and AEDSP. Overall, this study explored the inhibitory effects of Phoenix dactylifera L. seed extract on adipogenesis in 3T3-L1 cells on the molecular level. In each case, the total cell number of probiotic increased after 24, 48 and 72 hr in contrast with the negative control (distilled water) in which the probiotic cell number increased only slightly. The seed constitutes on average between 13% and 15% of the date fruit weight (11). After 48 h incubation the distilled water failed to support further growth. Besbes, S., Blecker, C., Deroanne, C., Drira, N. E., Attia, H. Date seeds: chemical composition and characteristic profiles of the lipid fraction. All the fermentation assays in this study were carried out under aerobic environment conditions at 28°C in a shaking incubator (170 rpm). Obviously, all these finding pertain to seeds which sur­vived accidental storage followed by controlled germination and are not indicative of the upper survival time limit of properly stored seeds. y=-3, n=0: y-3: 1.02: Has the species become naturalized where grown? (2) and Wang, (3) the scientific criteria that must be met for functional food ingredients to be recognized and classified as prebiotics are that they (i) are able to escape digestion in the upper GIT and not absorbed (ii) are fermented by the intestinal microflora (iii) are a selective substrate that changes colonic bacteria composition by stimulating the growth of beneficial colon bacteria such as LactobacilliorBifidobacteriaspp, and inhibiting the growth of pathogenic bacteria like Enterobacteriaceaeand Clostridium spp. Given the results, distilled water supplemented with the date seed substrate was identified as a possible alternative medium to the MRS-Min supplemented with a date seed substrate, because it is a more economic option when translated to industrial scale. Author information: (1)Department of Biochemistry and Molecular Biology, Faculty of Pharmacy, Helwan University , Cairo , Egypt and. Seed - pre-soak for 24 hours in warm water and sow in containers. Dates (Phoenix dactylifera) were obtained from a local date market in Makkah, Saudi Arabia and kernels were separated from the date flesh by hand-pitting under hygienic conditions. These media were used in this study with the addition of the three different date seed products i.e.FGDSP, AEDSP and DFC. Growth condition (incubated aerobically with shaking at 28°C, for 96 h). The fruits are oval-cylindrical and bright red to yellow when ripe. Most studies performed on date seeds have investigated only their chemical composition. A good source of functional food ingredients, particularly prebiotics, is one that contains a large quantity of carbohydrates, especially non-digestible carbohydrates like dietary fibre. Content Guidelines 2. B., Sharif, F. A., Usda, A. R. S. Preliminary analysis and potential uses of date pits in foods. II. However, this result should be taken into account in further studies. Mandalari, G., NuenoPalop, C., Tuohy, K., Gibson, G. R., Bennett, R. N., Waldron, K. W., Bisignano, G., Narbad, A., Faulds, C. B. Menne, E., Guggenbuhl, N. Fn-type chicory inulin hydrolysate has a prebiotic effect in humans. Top 35 Interview Questions on Plant Physiology (With Answers). The ability of the probiotic micro-organisms to utilise the finely ground date seed as a carbon source was determined by counting the colony forming units during the fermentation period (sampling after 0, 24, 48, 72, and 96 hours incubation). Welcome to BiologyDiscussion! Gum obtained from the tree is used to treat diarrhea. Dates were collected randomly from tamr (fully ripe dates) batches at the end of the season with no preference to size, colour, appearance, or firmness. Date Seed Products. . Introduction. As shown in the table 4, the addition of 0.6 ml of AEDSP positively affects the total cell numbers of probiotic populations in the flasks of all the test media. An increase in the total cell numbers of the probiotic was also observed in the flasks containing FGDSP and AEDSP, and a pH decrease was also observed. An API 50 CHL test was carried to determine their sugar fermentation patterns. This may be due to the fact that the probiotics used the date seeds extract as a carbon source. In this perspectives, the fruit of the date palm (date), a popular food product in the Middle East,  has been claimed for hundreds of years for its nutritional and health benefits in human and animal uses.have been claimed for. The total microbial populations were less than those reported with the FGDSP used in the first fermentation assay. The first fermentation assay was conducted by using the FGDSP in the flasks. Seeds with life span ranging from 3 years to 15 years. Table 1.Different concentrations of tested FGDSP,AEDSP and DFC product (fermentation     media), using isolated same probiotic strain and growth conditions. The chemical constituents of date kernels have been studied by several researchers (12; 13; 14; 15; 16; 17; 18; 19). It was successfully sprouted in 2005 (Sallon et al, 2008). The main reasons to divide the date seeds intothree tested products in this study were: first, there was no available information so far about their biological effects on microbial growth, especially probiotics which was the main interest of this study. In addition, some sugars are also considered in this category, such as raffinose, lactose, and stachyose, and sugar alcohols, e.g., sorbitol and xylitol. The medium was also prepared in the laboratory in its modified form which did not contain a carbon source so that it could be supplemented with the date seed products e.g. The          results    shown   in         table    4      indicate     that     the DFC was   utilized       by      theprobioticLactobacillus. The results shown in table 3 indicate that MRS-C broth containing different concentrations of FGDSP (06, 0.15 and 0.6 g/30 ml) all support the growth of the probiotic culture. Phoenix canariensis Chabaud – Canary Island date palm : Species: Phoenix dactylifera L. – date palm : Species: Phoenix loureiroi Kunth – Loureir's date palm : Species: Phoenix reclinata Jacq. Before sharing your knowledge on this site, please read the following pages: 1. Hence, in general, reducing the pH values in this study was evidence to indicate that DFC from date seeds may be used as prebiotic. Answer Now and help others. L. Family - Arecaceae.. Common Names(s) - Date Palm. Seed longevity is important for agriculture and biodiversity conservation, and understanding the basis for the extraordinary longevity of seeds from botanical collections could help improve seed banking technology. They were all Gram positive, short rods, non-spore forming, catalase negative and oxidase negative. Abdelaziz DH(1), Ali SA, Mostafa MM. Inoculated plates were covered with a layer of the same medium to create microaerophilic condition. Just to be sure you can use a 1 pint measure (about 550 ml) of vermiculite, moistened with 30 ml (about two tablespoons) of water. In recent years, there have been reports of seeds of some plants retaining viability upto many thousand years and even upto 10000 years, but, using 14C-dated techniques most of such reports have been found to be anecdotal and not authentic. The date derivatives are rich in nutraceuticals and phytochemicals. The most surprising observation in this fermentation assay was the decrease in the pH values from 6.2 to 3.9 in distilled water, the same as MRS-Oxoid, MRS-C + 0.6g FGDSP, MRS-C +0.15g and MRS-C + 0.06g FGDSP(Figures 1 and 2). dactylifera L.) cultivars (Barhi, Khalas, Manifi, Rezeiz, Sulaj, and Sukkari) growing in Saudi Arabia and to compare them with conventional palm olein.The mean oil content of the seeds was about 7%. It is a monocotyledonous woody perennial plant belonging to the Arecaceae family, which contains over 200 genera and at least 3000 species (10). The total bacterial numbers in the negative control flask (distilled water) werecompared with the other flask containing DFC during the fermentation period. The viable count of the isolated LAB (probiotic) from Actimel yoghurt was 1.21×107 CFU/g. Attalia, A. M., Harraz, F. M. Chemical Composition of the pits of selected date palm cultivars grown in the Qassim region, Saudi Arabia. The objectives in this study were therefore firstly, to investigate the effects of the date palm (Phoenix dactylifera L.) seed products in two forms (i) finely ground date seeds powders (FGDSP) and (ii) the aqueous extract of date seed powder (AEDSP) on the growth of probiotic lactic acid bacteria (LAB) such as Lactobacillus paracaseisspparacasei. In such cases the seeds longevity can be increased to a considerable extent by keeping them under suitable storage conditions. The term “dietary fibre” is applied to all non-digestible polysaccharides. This study has shown that the two date palm substrates FGDSP and AEDSP can potentially be utilized as carbon sources suitable for bacterial fermentation. Seeds with life span varying from 15 years to 100 years or more. Changes in pH values during fermentation of the three date seed products: The starting (blue) and final(red) pH after (96 hrs) incubation for the three date seed product; DFC, FGDSP and AEDSP. The longest-lived example of a viable seed of known age is the date palm, Phoenix dactylifera L., of which an estimated 2000-year-old seed was germinated in 2005. Date seed is a good source of bioactive compounds such as phenolics with antioxidant activity. After their maturation the seeds do not remain viable indefinitely. What are the general characters of bryophytes? Phoenix dactylifera seeds ameliorate early diabetic complications in streptozotocin-induced diabetic rats. It is often dried and eaten raw or used as food sweetener. Nowadays, there is increasing interest in the investigation, identification and development of novel prebiotic compounds, to support the claim of using functional foods as an alternative approach for promoting health and reducing the risk of diseases (4, 5, 6, 7, 8). What is its function? Synonym(s) - NA. This study analyzes the properties associated with the date seed (Phoenix dactylifera L.) to evaluate its suitability and viability as a new biofuel. Potential prebiotic properties of almond (. Consequently, one of the current challenges in nutrition sciences is improving gut health, using the concept of functional foods as a health promoting tool. The API 50 CHL characterization was also conducted on the LAB laboratory stock culture and the API 50 CHL test confirmed the strain as being Lactobacilluscasei (Table 2). The most significant observation in this assay was that there were no changes in the pH values in the flasks of the MRS-Min + 0.6 ml AEDSP compared to the MRS-Min control flask despite the fact that an increase in the total bacterial number was observed. Effect of the aqueous ethanolic extract from Phoenix dactylifera parthenocarpic dates on MDA-MB-231 and MCF-7 cancer cell lines viability at 24 h (a) and 72 h (b). Date palm (Phoenix dactylifera L.) seeds of the Khalas variety were used in this trial.They were provided by the Al Foah Company-Emirates Dates factory, Al-Ain, United Arab Emirates. Pick out the large, pointed seed. In the other flasks the pH values decreased as the population number increased (Figure 7). Thus, exploitation of such waste is very important in date cultivation and to raise income to this sector, especially if that product has a potential use as functional food ingredient in countries where the date palm is grown, such as Middle Eastern countries including Kingdom of Saudi Arabia. Table 3.First fermentation assay: Enumeration (CFU /ml) of probiotic using differentamounts of finely ground date seed powder (FGDSP) as substrate. In the second fermentation assay, MRS was prepared without peptone, glucose, yeast extract and Lab- Lemco powder (MRS-Min medium used in second fermentation assay contains g/L): Sorbitan mono-oleate;1ml; Dipotassium  hydrogen Phosphate,2.0; Sodium Acetate,5.0; Triammonium citrate,2.0; Magnesium Sulfate 7H2O; 0.2 ; Magnesium Sulfate 4H2O; 0.05; pH 6.2 ± 0.2). The DFC presented the potential to be applied as a novel source of prebiotics, by increasing the population of Lactobacillus paracaseisspparacaseias probiotics in addition to decreasing the pH values.This research proposed that date seeds could potentially be utilized as a starting material to manufacture functional food ingredients especially as they are a common waste product, especially in Middle Eastern countries. This website includes study notes, research papers, essays, articles and other allied information submitted by visitors like YOU. Amer, W. M. Taxonomic and documentary study of food plants in Ancient Egypt. It was prepared by removing the glucose substrate from the MRS-Oxoid and adding the remaining ingredients listed on the MRS-Oxoid. An increase in the total numbers of viable bacteria in the FGDSP and AEDSP fermentations (Figure 9) confirmed that these two date seed products could provide a suitable carbon source to support microbial growth. 4 filter paper. Share Your PDF File MRS-C without glucose substrate; b : FGDSP: Finely ground date seeds powders. A 0.1 ml of the 10-1 sample were then surface plated in duplicate onto MRS agar and aerobically incubated at 28°C for 48 h. Furthermore, 0.1 ml of appropriately diluted samples of reference strains was pour-plated in duplicate on MRS agar and treated the same way as the probiotic sample during its incubation. FGDSP and AEDSP could be used as a carbon source for bacterial fermentations. The Date Palm tree (Phoenix dactylifera L) is one of the oldest cultivated plants and has been used by man for over 6000 years (9). It was carried out on six different samples, four samples that contained different concentrations of FGDSP in synthetic MRS (MRS-C). The positive and negative fermentation results were reported as described in the manufacturer’s handbook and the species identification made by a computer program which was used to analyzed the results, as recommended by the manufacturer (bioMerieux). The fruits are edible. The Jepson Manual: 1993; Hussein, A. S., Al-Haami, G. A., Khalil, Y. H. The use of dates and date pits in broiler starter and finisher diets. Rybka, S., Kailasapathy, K. Media for the enumeration of yoghurt bacteria. They are considered as waste material of the several date processing plants that produce date powders, date juice, date confectionery, date syrup, pitted dates, date chocolate and coated dates. This study analyzes the properties associated with the date seed (Phoenix dactylifera L.) to evaluate its suitability and viability as a new biofuel. Specifically, the viability of replacing the existing gas boiler in a university building in the south of Spain with a system of biomass boilers using this biomass was analyzed as a case study. The total viable count of the yoghurt was 1.21×107 CFU/ ml. The three fermentation assays were carried out following the same procedure and under the same conditions with the different tested substrates (FGDSP, AEDSP and DFC). One control flask containing 30 ml distilled water was also used. The seeds of most of the crop plants have usually very short life-span and belong to the first category. In general, an increase in the numbers of probiotic was reported in response to the addition of the date seed product (FGDSP) during the fermentation period (96 h), in addition to reducing the pH. Buy Phoenix Dactylifera, Date Palm - 0.5 kg Seeds and 6000+ more gardening products online. 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